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Luciferase reporter assays Hs578 cells were transfected in batches in 48-well plates using 2.5 ng ER36 or ER66 and 50 ng tk-ERE-luc vector per well, as well as ALDH1A1 wild-type/mutant promoter-Luc for 48 h

Compared to semaglutide options Semaglutide (branded as Ozempic and Wegovy for injection, Rybelsus for oral) is tirzepatide primary competitor

Research-grade lysine is chosen for laboratory experiments where high analytical purity (99%+) and specific certifications (e.g., ACS grade) are required to ensure reproducible results

This is to ideally prevent the possibility of nasal bleeding and dizziness